Degradation Kinetics of Lignocellulolytic Enzymes in a Biogas Reactor Using Quantitative Mass Spectrometry

The supplementation of lignocellulose-degrading enzymes can be used to enhance the performance biogas production in industrial plants. Since structural stability these enzyme preparations is essential for efficient application, reliable methods assessment are crucial. Here, a mass-spectrometric-based assay was established monitor enzymes, i.e., integrity proteins, anaerobic digestion (AD). analysis extracts Lentinula edodes revealed rapid degradation with an approximate half-life 1.5 h. observed low AD corresponded previous results obtained content. workflow easily adapted monitoring other formulations and provides platform evaluating effects additions AD, together characterization biochemical methane potential order determine biodegradability organic substrates.